🌿Biology for Non-STEM Majors Unit 10 – Biotechnology

What's Biotechnology?

• Biotechnology harnesses cellular and biomolecular processes to develop technologies and products
• Combines biology, chemistry, physics, engineering, and computer science to manipulate living organisms and biological systems
• Includes a wide range of applications such as genetic engineering, tissue culture, and bioinformatics
• Involves the use of living systems and organisms to develop or make products (pharmaceuticals, crops, etc.)
• Encompasses both traditional techniques (selective breeding) and modern advances (genetic engineering)
• Can be broadly categorized into red (medical), green (agricultural), white (industrial), and blue (marine) biotechnology
• Relies heavily on recombinant DNA technology which involves manipulating DNA molecules to create new genetic combinations

Key Concepts and Techniques

• Genetic engineering introduces new DNA into an organism, enabling the expression of novel traits
  • Recombinant DNA technology combines DNA molecules from different sources to create new genetic combinations
  • Transgenic organisms contain genes inserted from a different species
• Polymerase Chain Reaction (PCR) amplifies small segments of DNA for analysis or manipulation
  • Uses a heat-stable DNA polymerase enzyme to make numerous copies of a specific DNA segment
• DNA sequencing determines the precise order of nucleotides within a DNA molecule
  • Next-generation sequencing (NGS) enables rapid, high-throughput sequencing of entire genomes
• Bioinformatics applies computational tools to manage and analyze large amounts of biological data
• Cell culture involves growing cells under controlled conditions outside their natural environment
  • Stem cell research utilizes cells capable of developing into various cell types for regenerative medicine
• Fermentation uses microorganisms to produce desired substances (antibiotics, enzymes, etc.)
• Bioprocessing employs biological systems to manufacture products on an industrial scale

Historical Milestones

• 1919: Károly Ereky coins the term "biotechnology" to describe the production of products from raw materials using living organisms
• 1928: Alexander Fleming discovers penicillin, the first antibiotic produced by a fungus
• 1953: James Watson and Francis Crick elucidate the double helix structure of DNA
• 1973: Herbert Boyer and Stanley Cohen develop recombinant DNA technology, enabling the creation of the first genetically engineered organism
• 1982: Humulin, the first recombinant DNA drug (human insulin), is approved by the FDA
• 1983: Kary Mullis invents the Polymerase Chain Reaction (PCR) technique for amplifying DNA
• 1994: The U.S. FDA approves the first genetically modified food, the Flavr Savr tomato
• 1996: Dolly the sheep becomes the first mammal cloned from an adult cell
• 2000: The Human Genome Project publishes a draft sequence of the human genome
• 2010: Scientists create the first synthetic bacterial cell with a completely synthetic genome

Applications in Medicine

• Biopharmaceuticals are drugs produced using biotechnology methods (monoclonal antibodies, recombinant proteins, etc.)
  • Insulin, growth hormones, and blood clotting factors are manufactured using genetically engineered bacteria or yeast
• Gene therapy introduces functional genes into cells to replace defective or missing genes
  • Has the potential to treat genetic disorders such as cystic fibrosis and sickle cell anemia
• Personalized medicine tailors treatments to an individual's genetic profile for improved efficacy and reduced side effects
• Regenerative medicine utilizes stem cells to repair or replace damaged tissues and organs
  • Tissue engineering combines cells, scaffolds, and growth factors to create functional tissue constructs
• Molecular diagnostics detect and monitor diseases at the molecular level using biomarkers and genetic testing
• Vaccines produced using recombinant DNA technology (hepatitis B) or virus-like particles (human papillomavirus)
• Antibiotics, vitamins, and other metabolites are produced by genetically engineered microorganisms

Agricultural and Environmental Uses

• Genetically modified (GM) crops are engineered to exhibit desirable traits (pest resistance, drought tolerance, etc.)
  • Bt corn produces a bacterial toxin that protects against insect pests
  • Golden Rice is fortified with beta-carotene to address vitamin A deficiency in developing countries
• Marker-assisted selection uses genetic markers to identify and select plants or animals with desired traits
• Biopesticides are naturally occurring substances used to control pests (Bacillus thuringiensis)
• Biofuels are produced from renewable biomass sources (corn, sugarcane, algae)
  • Cellulosic ethanol is derived from non-food plant materials (switchgrass, wood chips)
• Bioremediation employs microorganisms to break down and remove pollutants from contaminated sites
  • Genetically engineered bacteria can degrade oil spills and detoxify industrial waste
• Biosensors use biological components (enzymes, antibodies) to detect and measure specific substances in the environment

Ethical Considerations

• Genetically modified organisms (GMOs) raise concerns about potential ecological and health risks
  • Unintended consequences may arise from the release of GMOs into the environment
  • Allergenicity and toxicity of GM foods are ongoing areas of research and debate
• Gene editing technologies (CRISPR-Cas9) have the potential for misuse, such as creating "designer babies"
  • Germline modifications can be passed down to future generations, raising ethical questions
• Biopatents on living organisms and genetic sequences may hinder research and innovation
• Equitable access to biotechnology products and benefits is a global challenge
  • Developing countries may lack the resources to develop or afford biotechnology solutions
• Animal welfare concerns arise from the use of animals in biotechnology research and production
• Public trust and understanding of biotechnology are essential for its responsible development and application

Future Directions

• Synthetic biology aims to design and construct new biological parts, devices, and systems
  • Creating organisms with minimal genomes to serve as "chassis" for engineering novel functions
• Genome editing technologies (CRISPR-Cas9) enable precise, targeted modifications to DNA sequences
  • Potential applications include treating genetic diseases, improving crop yields, and creating disease-resistant animals
• Microbiome research explores the role of microbial communities in human health and the environment
  • Manipulating the gut microbiome may help prevent or treat various diseases
• Nanobiotechnology integrates nanotechnology with biotechnology for targeted drug delivery and biosensing
• Organ-on-a-chip systems mimic human physiology for drug testing and disease modeling
• Biomanufacturing advances aim to scale up and optimize the production of biotechnology products
• Computational biology and big data analytics will drive the discovery of new drugs and therapies

Real-World Examples

• Genentech's Herceptin is a monoclonal antibody used to treat HER2-positive breast cancer
• Novozymes produces industrial enzymes for applications in food processing, biofuels, and detergents
• Monsanto's (now Bayer) Roundup Ready crops are genetically engineered to tolerate the herbicide glyphosate
• Impossible Foods uses genetically engineered yeast to produce a plant-based heme protein for their meatless burgers
• Oxitec's genetically modified mosquitoes aim to control the spread of mosquito-borne diseases (dengue, Zika)
• Ginkgo Bioworks engineers yeast to produce rare and expensive compounds (saffron, cannabinoids)
• Vertex Pharmaceuticals' Trikafta is a combination drug for treating the underlying cause of cystic fibrosis
• Regeneron's monoclonal antibody cocktail (REGEN-COV) is used to treat COVID-19 infections