Analytical Chemistry

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Amber glass vial

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Analytical Chemistry

Definition

An amber glass vial is a small container made of brown-tinted glass, specifically designed to protect sensitive substances from light exposure. The amber color helps to filter out harmful ultraviolet (UV) rays, preserving the integrity of the sample inside. This is crucial for the storage and preservation of certain chemicals and biological materials that can degrade or react negatively when exposed to light.

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5 Must Know Facts For Your Next Test

  1. Amber glass vials are especially important for storing light-sensitive compounds like vitamins and certain pharmaceuticals, as exposure to light can lead to their degradation.
  2. These vials come in various sizes, making them versatile for different sample volumes, from small laboratory samples to larger storage needs.
  3. Amber glass is chemically resistant, which helps prevent reactions between the vial and the stored substance, ensuring sample integrity.
  4. Proper sealing of amber glass vials is critical; using screw caps or rubber stoppers can further protect samples from contamination and evaporation.
  5. When using amber glass vials, it's essential to store them in a cool, dark place to maximize the lifespan of the stored samples.

Review Questions

  • How does the amber color of glass vials contribute to the preservation of light-sensitive samples?
    • The amber color of glass vials helps to filter out harmful ultraviolet (UV) light, which can degrade sensitive substances over time. By blocking these UV rays, amber glass vials protect the contents from photodegradation, ensuring that chemicals, pharmaceuticals, or biological materials remain stable and effective for longer periods. This quality makes them essential for properly storing light-sensitive samples in laboratories.
  • Evaluate the advantages of using amber glass vials over clear glass vials for the storage of certain chemicals.
    • Using amber glass vials offers significant advantages compared to clear glass vials when it comes to storing light-sensitive chemicals. The amber tint protects against UV light exposure that can lead to degradation and loss of potency. Additionally, amber glass is chemically resistant, which helps maintain sample integrity. This makes amber vials a preferred choice for substances like vitamins and some pharmaceuticals that require protection from both light and potential chemical interactions.
  • Create a protocol for the storage of light-sensitive samples in amber glass vials that maximizes sample integrity and longevity.
    • To maximize the integrity and longevity of light-sensitive samples in amber glass vials, first select appropriate vial sizes based on sample volume and ensure they are clean and dry before use. Next, fill the vials with the sample, leaving minimal headspace to reduce oxidation risk. Securely cap the vials with screw caps or rubber stoppers to prevent contamination and evaporation. Store the filled vials in a cool, dark environment away from direct light sources. Regularly check for any signs of degradation or contamination during storage, and label each vial with pertinent information such as sample type and date of storage.

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