1.1 Overview of proteomics and its importance in biological research

Proteomics is the study of proteins in cells and organisms. It goes beyond genomics by examining actual protein levels, interactions, and modifications. This field bridges the gap between genes and cellular functions, offering insights into how proteins shape biological processes.

Proteomics has wide-ranging applications in medicine, drug development, and environmental science. It helps identify disease markers, develop targeted therapies, and understand how organisms respond to their environment. These applications make proteomics a powerful tool for advancing scientific knowledge and improving human health.

Fundamentals of Proteomics

Definition of proteomics

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  Frontiers | Proteomics Approaches for Biomarker and Drug Target Discovery in ALS and FTD View original

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  Frontiers | Illuminating Spatial and Temporal Organization of Protein Interaction Networks by ... View original

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  Frontiers | Integrative Analysis of Membrane Proteome and MicroRNA Reveals Novel Lung Cancer ... View original

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  Frontiers | Proteomics Approaches for Biomarker and Drug Target Discovery in ALS and FTD View original

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  Frontiers | Illuminating Spatial and Temporal Organization of Protein Interaction Networks by ... View original

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• Proteomics entails comprehensive analysis of protein structure, function, and interactions within cells, tissues, or organisms
• Examines entire protein complement (proteome) revealing cellular processes and physiological functions
• Proteome dynamically shifts in response to environmental stimuli and varies between cell types and developmental stages (embryonic stem cells vs mature neurons)

Proteomics vs genomics and transcriptomics

• Genomics studies complete gene set while transcriptomics analyzes all RNA transcripts
• Proteomics bridges gaps by providing actual protein abundance data, revealing protein-protein interactions, and identifying post-translational modifications
• Integration of omics technologies offers holistic view of cellular processes and illuminates genotype-phenotype relationships (eye color genes vs actual pigment production)

Significance of post-translational modifications

• Post-translational modifications (PTMs) chemically alter proteins after synthesis (phosphorylation, glycosylation, ubiquitination)
• PTMs impact protein structure, function, enzyme activity, localization, and mediate protein-protein interactions
• Proteomics techniques identify and characterize PTMs crucial for understanding protein function in vivo (insulin activation via proteolytic cleavage)

Applications of Proteomics

Applications in biomedical research

• Biomarker discovery identifies protein indicators for diseases or biological states enabling early detection and prognosis (prostate-specific antigen for prostate cancer)
• Drug development involves target identification, studying drug-protein interactions, and evaluating efficacy and toxicity
• Disease diagnosis utilizes proteomic profiling of diseased vs healthy tissues to develop diagnostic tests (blood protein panels for liver function)
• Personalized medicine tailors treatments based on individual protein profiles
• Environmental proteomics studies protein changes in response to environmental factors (heat shock proteins in stressed plants)
• Food science analyzes protein content and quality in food products (gluten content in wheat)