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Acidic amino acids

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Biochemistry

Definition

Acidic amino acids are a class of amino acids characterized by their side chains that contain carboxylic acid groups, resulting in a negative charge at physiological pH. This property is crucial for protein structure and function, as these amino acids can participate in various biochemical interactions, such as ionic bonds and hydrogen bonding, influencing protein folding and activity.

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5 Must Know Facts For Your Next Test

  1. The two primary acidic amino acids are aspartic acid and glutamic acid, both of which have side chains containing additional carboxyl groups.
  2. At physiological pH (around 7.4), acidic amino acids typically exist in their deprotonated form, giving them a net negative charge.
  3. These amino acids can play significant roles in enzyme active sites, influencing catalysis and substrate binding through their charged side chains.
  4. Acidic amino acids can participate in various interactions with basic amino acids, contributing to the overall stability of protein structures through salt bridges.
  5. Deficiencies or mutations involving acidic amino acids can lead to significant metabolic disorders, highlighting their importance in biochemical pathways.

Review Questions

  • How do acidic amino acids influence protein folding and stability?
    • Acidic amino acids influence protein folding and stability through their negatively charged side chains, which can engage in ionic bonds with positively charged basic amino acids. This interaction helps stabilize the three-dimensional structure of proteins. Additionally, these amino acids can form hydrogen bonds and participate in various biochemical interactions that are critical for maintaining proper protein conformation.
  • Discuss the significance of the pKa values of acidic amino acids in relation to their behavior in physiological environments.
    • The pKa values of acidic amino acids are significant because they determine the protonation state of the carboxylic acid groups at physiological pH. Since acidic amino acids have low pKa values, they tend to lose protons and exist as negatively charged zwitterions in biological systems. This characteristic impacts their interactions with other molecules and their role in enzymatic reactions, influencing both structure and function within proteins.
  • Evaluate the role of acidic amino acids in metabolic pathways and how alterations to these pathways might lead to disease.
    • Acidic amino acids play crucial roles in various metabolic pathways, including neurotransmitter synthesis and urea cycle metabolism. Any alterations or deficiencies in these pathways, such as reduced levels of aspartic acid or glutamic acid, can lead to disorders like hyperammonemia or neurological issues due to impaired neurotransmission. Understanding the function of these amino acids helps researchers develop targeted therapies for conditions related to their metabolic pathways.

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